Purification and biochemical characterisation of theEcoR124 type I modification methylase
نویسندگان
چکیده
منابع مشابه
DNA-methylase from regenerating rat liver: purification and characterisation.
DNA methylase has been purified 660-fold from nuclei from regenerating rat liver. The enzyme is able to methylate single stranded (ss) and double stranded (ds) DNA, the only reaction product being 5-methylcytosine. Previously unmethylated double stranded DNA from prokaryotes (M.luteus) as well as from eukaryotes (Ascaris suis) can serve as substrates. The synthetic copolymers (dG-dC)n . (dC-dG)...
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Ricin is a highly toxic plant toxin of Ricinus comtnunis seeds, commonly known as castor seeds. The toxin was extracted and purified using affinity and size exclusion chromatography. The purity of ricin was evaluated by the sodium dodecylsulphate-polyacrylamide gel electrophoresis. Purified ricin gives a single band under non-reduced condition and two bands under reduced condition. The molecula...
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چکیده ندارد.
The cloning, purification and characterization of the Eco RV modification methylase.
The gene for the Eco RV methylase has been cloned into a plasmid under control of the strong lambda PL promoter and overexpressed in E. coli. This plasmid, pVIC1, gives reliable overexpression of the methylase at levels of about 20% of total protein. Maximum yields of soluble protein are achieved after about 6 hours of induction. If the cells are harvested later than this much of the enzyme is ...
متن کاملThe deoxyribonucleic acid modification and restriction enzymes of Escherichia coli B. I. Purification, subunit structure, and catalytic properties of the modification methylase.
The modification methylase of Escherichia coli B has been purified to apparent homogeneity. The enzyme can exist in several forms, each possess;ng two nonidentical polypeptides, /3 and y, of molecular weights of 60,000 and 55,000, respectively. Freshly isolated enzyme has the structure plyI, but upon storage at neutral pH and low salt, it disproportionates, producing another form, fl3~1. Treatm...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.2.179